Fig. 6. The functions of endothelial cells was mediated by the knockdown of Gpr137b-ps in response to OGD/R. (A) Knockdown of Gpr137b-ps reduced the viability of endothelial cells after OGD/R. (B-E) Knockdown of Gpr137b-ps reduced the capacity for migration of endothelial cells after OGD/R according to the scratch-wound and transwell assays. (F) Knockdown of Gpr137b-ps reduced the number of loops of endothelial cells after OGD/R according to the tube formation assay. (G) Statistical analysis of the capacity for migration in cells treated with shGpr137b-ps compared with a non-targeting Gpr137b-ps shRNA (* p <0.05, ** p <0.01, *** p <0.001 vs. non-targeting Gpr137b-ps shRNA). (H) Transfection with Gpr137b-ps prevented the elevation of CD31 levels in endothelial cells after OGD/R. (I) Summary graph depicting the CD31 signal intensity, which was significantly decreased after treatment with shGpr137b-ps compared with treatment with a non-targeting shRNA. (* p <0.05, ** p <0.01, *** p <0.001 vs. non-targeting Gpr137b-ps shRNA). All results are presented as the mean ± SEM.